Chemycal Evolution
Tonight the true process of evolution by Bio-Chemical means begins with our yeast RL-1.2- Lazarus. The yeast becomes hardier and more viable by acclimitizing it to other types of sugar. In this case that sugar is Amylase. While simple table sugar is essentially glucose
Here is our starch not yet dehydrated
We here at Lab 5 strive by a deeper standard and so introduce a different kind of sugar. One that has been broken down from potatoes starch
To Amylase
A catalytic enzyme meant to break down starch to Amylase a sugar molecule a precursor to glucose, a simple sugar . Simple sugar is easiest for yeast to assimilate however different types of sugar give the Yeast different genetic characteristics over time.
Above completed yeast with nutritional supplements and amylose.
Vital Statistics Prior to Chemical Alteration:
Incubator temp: 24.1C
Diff s/y: -0.9C
Vessel temp: 22.5C
Diff s/y: -1.1C
Yeast temp: 24.8C
Diff s/y: +1.7C
Brix:13.1%
Diff s/y:+1.9%
Sg:1.053
Diff s/y: +0.008
pH: 4.8
Diff s/y:0
Chemical Process to extract Starch from potatoes and extract Amylose:
1.peel 3 Russet potatoes
2.grate potatoes into bowl with water - ensuring water remains above potatoes.
Mix thoroughly until clear liquid becomes milky. Cover with a plate to avoid contamination.
Shouldn't have to tell you this but wash your hands a priori- or first and keep hands clean at all times
3.allow to sit for 30 minutes starch will settle at bottom.
Filter liquid don'tpour out white residue on buttom of vessel.
Add liquid back to filtered solid
This is called washing as it enables more starch to be washed out of potatoe.
4.allow to sit another 30 minutes repeat this wash phase one more time.
5. White powdery substrate forms on the bottom.
Unfiltered starch waiting to be dehydrated via toaster oven at 75 C
100 ML water is added to a container with a lid.
A pinch from the starch sample is added to each test vessel
Process is repeated for 2nd sample: this is the control sample or calibration sample
3-4 drops of 5% iodine solution are added to each and stirred
Spit 3 times in non control sample.
Wait 30 mins look for colour change reaction - should turn clear when starch has been converted to simple sugar.
Process is duplicated on control sample after to verify findings.
Sample bath!