Bion-Innoculated Yeast Culture Lab: Bio Archemy

We've been on a creative break for a while here at Laboratory 5 but interestingly even while taking a break some upgrades to the NGBD were put into effect this includes the addition of the stealth uhf/vhf/fm antenna

and also the addition of another antenna located directly on the tower itself

some testing was performed using these new pieces of hardware and we were far more than happy with the results!! Moving on it was time to get back to the work at hand as inscribed above the Alchemyst of Olde's furnace or athanor was the one solitary word:

Now back to breeding our extraordinary yeast!

But "Why yeast?" you might ask us.

Quite simply put when it comes to manufacturing medicines every step of the process is just as important from the most insignificant (read microcosm) to the largest (macrocosm) everything counts nothing can be taken for granted.

So we start with that which produces extracts the medicine the yeast itself and we work our way through the process that way.

So first we make the yeast:

Step 1: sterilize!

Start with the wash phase- I would use antibacterial detergent just to ensure that your washing procedure is as thorough as possible.

Rinse with as hot of water as you can attain. My rinse water was consistently 64.6 degrees celcius.

Sanitize Phase

1 cap full of bleach to about 1/2 sink full of water should suffice.

You'll be sterlizing the following materials

inoculation vessel

mortar and pestle

a stainless steel spoon

temperature probe- and it's scabbard

magic bullet container for blending

(you'll have to wipe down the blades...etc. with disposable wipes and rinse never leave the cleaning solution on contact surfaces

You'll need the following as well

scale accurate to 0.1g (atleast)

ocular refractometer

pH strips (can be purchased in the pool section)

disposable gloves

disposable wipes

bleach

1 bag of granulated white sugar

(definitely optional: distilled and refined pure fructose I used it so I have to include it here: basically just distill almost all water out of fruit juice to obtain the pure fructose syrup it's quite simple

Blender - I used a magic bullet it works freakin great lol

1 cup of grapes

1/2 cup of orange peels

concentrated Plant food 10-15-10

multi vitamin

dried, powdered bions from a former lab (I didn't post this process because it is proprietary)

Now that everything is sterilized we're going to clean out our first wash sink with bleach.

Rinse thoroughly then wipe it down with disposable antibacterial wipes.

Rinse Once more repeat for the other sink which will suffice for drying everything we've just sterilized.

Use the disposable wipes to clean and disinfect the surface you'll be preparing your yeast culture on prior to the next step as you're waiting for your sterilized equipment to air-dry. It's important to allow it to air-dry as you'll avoid contaminating your instruments with bacteria say from a drying towel.

8.Turn on your scale. Tare the Scale.

Wipe down the outside of the inoculation vessel with the disposable wipes. do not wipe the top lip or the inside The water from the final rinse phase should be mostly evaporated a little remaining won't affect things.

10.

Wipe down the scale with disposable wipes.

(in this instance it's okay to leave very small traces of cleaning solution as this will leave a protective barrier)

11.

Weigh the container or vessel (mine was 545 g). Tare your scale. It's ok if it deactivates and you don't technically need to tare the scale again I just have mild OCD lmao.

12.

Now you're going to add your grapes and your orange peels. Fill up to 3/4 of total volume leaving some headspace.

13.

Blend for 60s.

14.

add the frothy solution to your vessel and close the lid firmly this will help avoid contamination from the air and without the container itself.

15.

Grind up a multivitamin in the mortar and pestle.

16.

Add the vitamin to the yeast culture.

17.

Add 8 drops of the concentrated plant food to the culture.

18.

Add 2 tspn's of white granulated sugar to your culture.

19.

Sterlize your spoon in the following way:

a. rinse off sugar

b. sterilize with disposable wipe (allow cleaning agent to remain on the surface 20-25s)

c.rinse with hot water

d. air dry by shaking in a separate room to rid excess water and to cool down the surface.

20. (optional step)

add 2 tspns of the purified fructose sugar

21.

Stir your yeast culture (if you added purified fructose sugar syrup ensure you perform step 19 between additions and stirring).

22.

take initial temperature of yeast (it must be within the range of 20-27 degrees celcius it can be a bit higher but higher than 30 degrees will cause it to ferment and since we're gathering and evolving our yeast strain that is a most undesirable result). My temperature was 28.9 degrees celcius.

23.

Take final volume weight by turning your scale on (unless it's still on). If you turn your scale on the vessel will read "0" which is fine since you would have to tare it again anyway. My final volume was 304g.

24.

Optical Refractometer Test

(I would highly recommend you purchase one of these !)

here's a helpful article about refractometers:

(https://en.wikipedia.org/wiki/Refractometer)

10% Brix / 1.040 SG with potential alcohol yield of 5.2% (not much yield there but it will get better as our strain evolves).

25.

pH Test

(follow the directions as you would for water)

I performed 2 tests both of which confirmed a pH of 4.8 which is ideal conditions for our yeast too much higher in the neutral to alkaline range and we would grow mold instead!

And that's about it! The yeast culture is prepared now we can move on in our next lab to incubating the yeast in a bion rich environment.

I never said this was a glamor shot :-P but this is what the yeast will typically look like at first. Much more to come stay tuned!